Journal: Applied and Environmental Microbiology

Article Title: Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis

doi: 10.1128/AEM.00178-19

Figure Lengend Snippet: Strains used in this work

Article Snippet: Therefore, the sporulation pathway does not significantly influence the B. subtilis fungal adaptation under our experimental conditions. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain Relevant phenotype and/or genotype Comment(s) and/or source b (reference) F. verticillioides Wild-type isolate CEREMIC a B. subtilis NCIB3610 Wild-type Marburg isolate, surfactin (Srf) proficient and biofilm formation (Bio) proficient Laboratory collection ( 18 ) MR101 JH642 amyE ::P ctc-lacZ :: cat Laboratory collection ( 31 ) DG555 amyE ::P ctc-lacZ :: cat , similar to NCIB3610 but also has a reporter of SigB activity This work, MR101→NCIB3610 DG5572 JH642 Δ rsbU :: kan Laboratory collection ( 30 ) DG5573 JH642 ΔrsbP :: spc Laboratory collection ( 30 ) DG5574 JH642 ΔrsbUP :: kan-spc Laboratory collection ( 30 ) DG556 Δ rsbU :: kan , similar to NCIB3610 but deficient in the environmental pathway of SigB activation This work, DG5572→NCIB3610 DG557 Δ rsbP :: spc , similar to NCIB3610 but deficient in the energy-related pathway of SigB activation This work, DG5573→NCIB3610 DG558 Δ rsbUP :: kan-spc , similar to NCIB3610 but deficient in the energy-related and environmental pathways of SigB activation This work, DG556→DG558 MR644 JH642 Δ sigB :: neo Laboratory collection ( 31 ) DG559 Δ sigB :: neo , similar to NCIB3610 but deficient in SigB activity (strain sensitive to stress) This work, MR644→NCIB3610 DG560 NCIB3610 Δ srfAA :: ery , idem to NCIB3610 but deficient in surfactin synthesis (also deficient in biofilm formation) Laboratory collection ( 18 ) MR760 JH642 amyE ::P srf-lacZ :: cat Laboratory collection ( 18 ) DG561 amyE ::P srf-lacZ :: cat , similar to NCIB3610 but also has a reporter of surfactin production This work, MR760→NCIB3610 DG562 Δ sigB :: neo amyE ::P srf-lacZ :: cat This work, DG559→DG561 DG563 Δ rsbU :: kan amyE ::P srf-lacZ :: cat This work, DG556→DG561 DG564 Δ rsbP :: spc amyE ::P srf-lacZ :: cat This work, DG557→DG561 Open in a separate window a Centro de Referencia en Micología, Universidad Nacional de Rosario. b Donor DNA→receptor strain.

Techniques: Activity Assay, Activation Assay

Journal: Applied and Environmental Microbiology

Article Title: Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis

doi: 10.1128/AEM.00178-19

Figure Lengend Snippet: Biocontrol proficiency of PGPR B. subtilis: in vivo roles of SigB and surfactin. (A and B) Germination efficiency (A) and plant growth (root length) (B) of Zea mays infected with F. verticillioides in the absence or presence of B. subtilis (see Materials and Methods for details). A typical output of three independent experiments is shown. (C) A cartoon summarizing the beneficial interactions between surfactin-producing PGPR B. subtilis cells and plants to resist phytopathogenic fungi. For simplicity, the stimulatory effect of plant polysaccharides on biofilm formation and surfactin synthesis is not indicated (see the text for details).

Article Snippet: Therefore, the sporulation pathway does not significantly influence the B. subtilis fungal adaptation under our experimental conditions. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain Relevant phenotype and/or genotype Comment(s) and/or source b (reference) F. verticillioides Wild-type isolate CEREMIC a B. subtilis NCIB3610 Wild-type Marburg isolate, surfactin (Srf) proficient and biofilm formation (Bio) proficient Laboratory collection ( 18 ) MR101 JH642 amyE ::P ctc-lacZ :: cat Laboratory collection ( 31 ) DG555 amyE ::P ctc-lacZ :: cat , similar to NCIB3610 but also has a reporter of SigB activity This work, MR101→NCIB3610 DG5572 JH642 Δ rsbU :: kan Laboratory collection ( 30 ) DG5573 JH642 ΔrsbP :: spc Laboratory collection ( 30 ) DG5574 JH642 ΔrsbUP :: kan-spc Laboratory collection ( 30 ) DG556 Δ rsbU :: kan , similar to NCIB3610 but deficient in the environmental pathway of SigB activation This work, DG5572→NCIB3610 DG557 Δ rsbP :: spc , similar to NCIB3610 but deficient in the energy-related pathway of SigB activation This work, DG5573→NCIB3610 DG558 Δ rsbUP :: kan-spc , similar to NCIB3610 but deficient in the energy-related and environmental pathways of SigB activation This work, DG556→DG558 MR644 JH642 Δ sigB :: neo Laboratory collection ( 31 ) DG559 Δ sigB :: neo , similar to NCIB3610 but deficient in SigB activity (strain sensitive to stress) This work, MR644→NCIB3610 DG560 NCIB3610 Δ srfAA :: ery , idem to NCIB3610 but deficient in surfactin synthesis (also deficient in biofilm formation) Laboratory collection ( 18 ) MR760 JH642 amyE ::P srf-lacZ :: cat Laboratory collection ( 18 ) DG561 amyE ::P srf-lacZ :: cat , similar to NCIB3610 but also has a reporter of surfactin production This work, MR760→NCIB3610 DG562 Δ sigB :: neo amyE ::P srf-lacZ :: cat This work, DG559→DG561 DG563 Δ rsbU :: kan amyE ::P srf-lacZ :: cat This work, DG556→DG561 DG564 Δ rsbP :: spc amyE ::P srf-lacZ :: cat This work, DG557→DG561 Open in a separate window a Centro de Referencia en Micología, Universidad Nacional de Rosario. b Donor DNA→receptor strain.

Techniques: In Vivo, Infection

Journal: Scientific Reports

Article Title: Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro

doi: 10.1038/s41598-018-30446-1

Figure Lengend Snippet: Effects on tube formation in HUVECs treated with exosomes released from PK-45H cells. Tube formation assay was performed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Cosmo Bio). A dynamin-dependent exosomal endocytosis promoted tube formation in HUVECs. Data are expressed as means ± standard deviations (each n = 3). Statistical significance ( P ≤ 0.05) was examined with the Tukey–Kramer test for multiple comparisons.

Article Snippet: Tube formation assay was performed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Cosmo Bio).

Techniques: Tube Formation Assay, In Vitro, Angiogenesis Assay